Induction of Somatic Embryogenesis in Saffron Using Thidiazuron (TDZ)
نویسندگان
چکیده
منابع مشابه
Morphoregulatory role of thidiazuron : substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium hypocotyl cultures.
Somatic embryogenesis was induced in hypocotyl explants of geranium (Pelargonium x hortorum) cultured on media supplemented with various concentrations of N-phenyl-N'-1,2,3-thiadiazol-5-ylurea (thidiazuron). In less than 2 weeks, somatic embryos were observed in treatments containing levels of thidiazuron (TDZ) ranging from 0.2 to 1.0 micromolar. The use of N(6)-benzylaminopurine in combination...
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Induction of somatic embryogenesis in absence of exogenous auxin can clarify some basic concepts of somatic embryogenesis. Explants from leaf and petiole of Cucumber (Cucumis sativus) were used in this experiment for somatic embryogenesis using three different modified B5 basal media; boron and 2,4-D but no IAA as control (I), boron and IAA but no 2,4-D (II) and no boron, 2,4-D and IAA (III). ...
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Somatic embryogenesis was initiated in C. sativus L. from shoot meristems on LS medium containing BAP (2×10-5 M)+NAA (2×10-5 M). Various stages of somatic embryogenesis were observed in the same medium and the development was asynchronous. Somatic embryo development proceeded through well recognized sequences (globular to embryoids) with clearly discernible bipolar regions. Matured embryos coul...
متن کاملThidiazuron induced somatic embryogenesis in Coffea arabica L. and Coffea canephora P ex Fr
The rapid direct and repetitive somatic embryogenesis in Coffea arabica and C. canephora genotypes was tested on Murashige and Skoog medium (MS) containing thidiazuron (TDZ) (1-phenyl-3-(1,2,3,-thiadiazol-5-yl)urea) in concentrations of 2.27–11.35s M. Segments taken from cotyledon leaf, first leaf and stalk of regenerated plantlets produced clusters of somatic embryos directly from cut portions...
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ژورنال
عنوان ژورنال: Pakistan Journal of Biological Sciences
سال: 2007
ISSN: 1028-8880
DOI: 10.3923/pjbs.2007.3564.3570